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Synthego Inc ribonucleoprotein rnp complexes
Ribonucleoprotein Rnp Complexes, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnp+complex/complexes+rnp/bio_rxiv__64898__2026__04__29__720127-117-20-38
Average 86 stars, based on 1 article reviews
ribonucleoprotein rnp complexes - by Bioz Stars, 2026-10
86/100 stars

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Article Title: Signaling via a CD27-TRAF2-SHP-1 axis during naive T cell activation promotes memory-associated gene regulatory networks.
Article Snippet: Per single reaction, 2x106 freshly isolated Tn cells were washed twice with pre-warmed PBS and resuspended in 20 ml of P3 buffer (Lonza, V4XP-3032).

Article Title: CRISPR-Cas9 engineering of human T regulatory cells - Design and optimization of a manufacturing process.
Article Snippet: CRISPR KO: For the formation of an RNP complex synthetic single guide RNA (sgRNA) (Synthego) was brought to 200 μM concentration by reconstitution in sterile TE buffer followed by 15 min incubation with recombinant NLS-SpCas9 protein (IDT; 1081059) at 37◦C at a Cas9:RNA ratio of 1:2.5 (Table S13).

CRISPR:

Article Title: SMPD4 -mediated sphingolipid metabolism regulates brain and primary cilia development
Article Snippet: .. CRISPR guides in an RNP complex designed by Synthego were transfected into control iPSCs. ..

Article Title: Signaling via a CD27-TRAF2-SHP-1 axis during naïve T cell activation promotes memory-associated gene regulatory networks
Article Snippet: .. To form the CRISPR-Cas9-sgRNA RNP complex, synthetic SHP-1 sgRNAs (Synthego) were incubated with Cas9 nuclease protein NLS (Horizon Discovery, Cat# CAS12206 ) at a ratio of [4:1] (160pmol sgRNA+ 40pmol Cas9) for 15 min at 37 °C and then kept at 4 °C until further processed. .. Per single reaction, 2×10 6 freshly isolated Tn cells were washed twice with pre-warmed PBS and resuspended in 20 μl of P3 buffer (Lonza, V4XP-3032).

Transfection:

Article Title: SMPD4 -mediated sphingolipid metabolism regulates brain and primary cilia development
Article Snippet: .. CRISPR guides in an RNP complex designed by Synthego were transfected into control iPSCs. ..

Article Title: Replication-associated base excision repair/single-strand break repair regulates PARG inhibitor response via the PRMT1/PRMT5/ATR axis
Article Snippet: .. Cells were seeded at a density of 2 × 10 5 cells/well in a six-well plate and incubated for 24 h. The non-viral ribonucleoprotein (RNP) transfection complex, purchased from Synthego Corporation, was prepared by combining three single guide RNAs (sgRNAs) targeting an early exon of each respective gene— PARP2, XRCC1, POLB, APTX , or LIG3— with purified Cas9 protein and the CRISPRMAX-Cas9 transfection reagent in serum-free OptiMEM. ..

Control:

Article Title: SMPD4 -mediated sphingolipid metabolism regulates brain and primary cilia development
Article Snippet: .. CRISPR guides in an RNP complex designed by Synthego were transfected into control iPSCs. ..

Concentration Assay:

Article Title: CRISPR/Cas9 Knockout of Shell Matrix Protein 1 in the Slipper-Snail Crepidula atrasolea.
Article Snippet: .. To prepare the RNP complex, synthetic guides (Synthego) were resuspended with nuclease‐free water, from their lyophilized state, to make a stock concentration of 100 μM. ..

Incubation:

Article Title: Signaling via a CD27-TRAF2-SHP-1 axis during naïve T cell activation promotes memory-associated gene regulatory networks
Article Snippet: .. To form the CRISPR-Cas9-sgRNA RNP complex, synthetic SHP-1 sgRNAs (Synthego) were incubated with Cas9 nuclease protein NLS (Horizon Discovery, Cat# CAS12206 ) at a ratio of [4:1] (160pmol sgRNA+ 40pmol Cas9) for 15 min at 37 °C and then kept at 4 °C until further processed. .. Per single reaction, 2×10 6 freshly isolated Tn cells were washed twice with pre-warmed PBS and resuspended in 20 μl of P3 buffer (Lonza, V4XP-3032).

Article Title: Replication-associated base excision repair/single-strand break repair regulates PARG inhibitor response via the PRMT1/PRMT5/ATR axis
Article Snippet: .. Cells were seeded at a density of 2 × 10 5 cells/well in a six-well plate and incubated for 24 h. The non-viral ribonucleoprotein (RNP) transfection complex, purchased from Synthego Corporation, was prepared by combining three single guide RNAs (sgRNAs) targeting an early exon of each respective gene— PARP2, XRCC1, POLB, APTX , or LIG3— with purified Cas9 protein and the CRISPRMAX-Cas9 transfection reagent in serum-free OptiMEM. ..

Purification:

Article Title: Replication-associated base excision repair/single-strand break repair regulates PARG inhibitor response via the PRMT1/PRMT5/ATR axis
Article Snippet: .. Cells were seeded at a density of 2 × 10 5 cells/well in a six-well plate and incubated for 24 h. The non-viral ribonucleoprotein (RNP) transfection complex, purchased from Synthego Corporation, was prepared by combining three single guide RNAs (sgRNAs) targeting an early exon of each respective gene— PARP2, XRCC1, POLB, APTX , or LIG3— with purified Cas9 protein and the CRISPRMAX-Cas9 transfection reagent in serum-free OptiMEM. ..



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